Figure 3 | Scientific Reports

Figure 3

From: High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment

Figure 3

Low-throughput synthesis of gene fragments. (a) Selective amplification of each subpool for generation of lycopene biosynthetic genes. Subpools were directly amplified using the oligo pool cleaved from the microchip as the template. (b) PCR of each error-depleted subpool. The oligo subpool was amplified with the error-depleted elute from etMICC as the template. (c) Fragments assembled by LCR from error-depleted oligo subpools. M, 20 bp DNA ladder (TaKaRa Bio). Red arrows indicate target fragments. OLS, oligo subpool; ER, error-removed.

Back to article page