Figure 3
From: Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging
Live cell imaging using Pink Flamindo with photoactivated adenylyl cyclase, and dual-colour imaging with G-GECO. (a) Sequential images and time course of fluorescence intensity of Pink Flamindo expressed in HeLa cells after consecutive exposure to 100 μM Fsk, 200 μM IBMX and 100 μM DDA. Scale bar represents 20 μm. The data represent the means ± standard deviation (n = 30 cells from 5 experiments). (b) Sequential images and time course of fluorescence intensity of MIN6 m9 cells expressing Pink Flamindo during the application of 25 mM glucose. Scale bar represents 20 μm. The data represent the means ± standard deviation (n = 15 cells from 4 experiments). (c) Sequential images and time course of fluorescence intensity of HeLa cells co-overexpressing Pink Flamindo and photoactivated adenylyl cyclase (bPAC) upon blue light laser excitation at 1.8 μW. Scale bar represents 20 μm. The data represent the mean ± standard deviation (n = 23 cells from 4 experiments). (d) Sequential images of MIN6 m9 cells co-overexpressing Pink Flamindo and G-GECO after consecutive exposure to 200 μM tolbutamide. Scale bar represents 20 μm. (e) Time course of fluorescence intensity of MIN6 m9 cells co-overexpressing Pink Flamindo and G-GECO. The data represent means ± standard deviation (n = 18 cells from 4 experiments).
