Figure 6 | Scientific Reports

Figure 6

From: Measuring the Poisson’s Ratio of Fibronectin Using Engineered Nanofibers

Figure 6

Quantifying the volumetric changes in strained FN nanofibers. (a) FN nanofibers were strained by releasing the FN nanofibers onto PDMS supports and uniaxially stretching them using micromanipulators. (b) An AFM scan of a ~67 μm wide FN nanofiber in a pre-release state on PIPAAm. The fiducial marks added an extra FN layer to the FN nanofibers and thus appear white in the AFM images. The volume was measured in the regions between fiducial marks (red outline). (c) After being strained to ~98%, the FN nanofiber was immobilized in the strained state and scanned using AFM. The volume was measured in the regions between fiducial marks (yellow dotted outline). (d) Quantification of FN nanofiber volume indicates there was no statistically significant difference.

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