Figure 3

Validation of selected proteins in LSECs from NaAsO₂-induced LSEC dedifferentiation and CCl₄-induced liver injury mice model. (A) Differential expressions of 2 up-regulated proteins (CLTC, Dab2) and 3 down-regulated proteins (Galectin-3, SAMHD1, Rab-10) were validated by Western blot. The relative ratios of proteins by iTRAQ analysis were shown right. All western blot experiments were repeated at least three times. Full-length blots are included in Supplemental Information. (B) Representative SEM images of liver sinusoids (n = 5) and quantitative porosity of sinusoidal fenestrae at 0 and 6^th week with CCl₄ administration. Bar = 1μm. (C) Masson’s trichrome staining and αSMA expression in liver at 6^th week with CCl₄ administration (n = 5) were analyzed by immunohistochemistry analysis, Bar = 100μm. (D) Western blot analysis of primary LSECs isolated from 0 and 6^th week for the levels of CLTC, Dab2, Galectin-3, SAMHD1 and Rab-10 proteins, and GAPDH was used as loading control. All western blot experiments were repeated at least three times. Full-length blots are included in Supplemental Information. (E) CLTC expression in liver at normal control mice and mice at 6^th week with CCl₄ administration (n = 5) were analyzed by immunohistochemistry analysis, Bar = 100μm.