Figure 1 | Scientific Reports

Figure 1

From: ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages

Figure 1

NLRC4 activates a caspase-1-independent cell death pathway in absence of TLR signaling. (a–c) BMDMs with or without Pam3CSK4 (1 μg ml−1) pre-stimulation were electroporated with flagellin (0.5 μg ml−1). (a) LDH release measured after 16 h. Data is represented as mean ± SD; n = 3. (b) % YOYO-1 positive BMDMs from two mice/genotype after flagellin electroporation. Live cell images taken every 30 min up to 16 h. (c) Transmission electron microscopy BMDMs 6 h after flagellin electroporation. Representative images captured at 1000× magnification or 2000× magnification. Black arrows (→) indicate free nuclei. White asterisks (*) indicate chromatin condensation.

Back to article page