Table 1 Cleavage timing, embryo collection time and number of cells in Day 2 and Day 3 embryos used for single-cell transcriptome profiling.

From: Single-cell RNA sequencing reveals developmental heterogeneity of blastomeres during major genome activation in bovine embryos

Embryo designation

1st cleavage (hpf)

2nd cleavage (hpf)

3rd cleavage (hpf)

Embryo collection (hpf)

Total cell number

Day2-E1

25:45

33:51

41:15

44:00

8*

Day2-E2

27:01

34:06

42:50

44:00

9

Day2-E3

27:01

34:26

39:45

44:00

9

Day2-E4

27:11

35:11

42:10

45:00

8

Day2-E5

27:35

34:25

45:25

45:30

8

Day2-E6

27:45

37:30

45:04

45:04

6

Day3-E1

29:18

38:45

47:07

67:00

15

Day3-E2

26:08

34:23

41:17

67:00

16

Day3-E3

27:48

35:53

44:02

67:00

16

Day3-E4

25:53

33:45

40:47

67:00

13

Day3-E5

29:56

37:16

45:20

69:00

17

Day3-E6

29:51

37:31

45:15

69:00

14

Day3-E7

27:15

36:20

44:15

71:00

16

Day3-E8

25:35

33:45

39:56

71:00

16

  1. In vitro developing embryos were observed by time-lapse microscopy, and embryos with high developmental potential were selected based on the timing (hours post fertilization; hpf; shown as hours:minutes) of the first three cleavage divisions. *1 cell was lost during the cell collection.
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