Figure 4
Cytokines and chemokines attracting T and B cells are upregulated in kidneys during development of TLS and nephritis. The total kidney gene expression of (a) LTβ (n = 24, 27 and 26), (b) LTβR (n = 24, 24 and 26), (c) VCAM1 (n = 35, 28 and 14), (d) ICAM1 (n = 23, 11 and 6), (e) CCL19 (n = 18, 19 and 26) (f) CXCL13 (n = 24, 28 and 27) were analyzed by qPCR. mRNA expressions are shown as FC mean ± SEM compared to young 7 wo mice. Kruskal-Wallis test with Dunn’s Multiple Comparison Test. *P < 0.05; **P < 0.01; ****P < 0.0001. Gene expression in isolated TLS and kidney tissue from proteinuric mice was normalized against average ΔCT of all kidney tissue samples (g–l). The gene expression in TLS and kidney tissue was also normalized to young 7 wo mice (second panel in g–l).
