Figure 1

MHC-I and sodium-potassium ATPase-subunits are broadly expressed across different cell types. (A) The two surface proteins b2m as part of the MHC-I complex and CD298, a subunit of the sodium potassium pump, were selected as potential targets for live cell barcoding. (B) Human whole blood was subjected to red blood cell (RBC) lysis and subsequently stained with heavy-metal isotope-conjugated antibodies for mass cytometry (Table S1). Cells were pre-gated on live, single, CD45⁺ and CD235ab^-. The main immune lineages were then identified via the indicated gating scheme. (C) Expression of b2m (light blue, left), CD298 (magenta, middle) and combined expression values from b2m and CD298 using the same reporter isotope (dark blue, right) on immune populations as in B. (D) Expression of b2m (light blue, left), CD298 (magenta, middle) and combined expression values from b2m and CD298 using the same reporter isotope (dark blue, right) on various cancer cell lines. Shown is one representative experiment out of two.