Table 1 Combination analysis for the cytotoxicity of TQ and GCB against MCF-7 and T47D breast cancer cell lines.

From: Thymoquinone synergizes gemcitabine anti-breast cancer activity via modulating its apoptotic and autophagic activities

Exposure time

MCF-7

T47D

24 h

IC50 (µM)

R-value (%)

IC50 (µM)

R-value (%)

GCB

32.6 ± 3.6

2.7 ± 0.6

7.6 ± 0.7

6.5 ± 0.5

TQ

80.1 ± 9.8

55.5 ± 1.2

>100

N/A

GCB + TQ

3.4 ± 1.3

0.1 ± 0.03

0.3 ± 0.02

4.3 ± 0.2

CI-value

Synergism/0.53

Synergism/less than 0.34

48 h

GCB

32.2 ± 1.1

0.5 ± 0.1

6.4 ± 1.4

2.3 ± 0.5

TQ

32.7 ± 1.1

1.2 ± 0.5

104 ± 3.7

N/A

GCB + TQ

3.6 ± 0.7

0.9 ± 0.1

0.27 ± 0.07

1.8 ± 0.4

CI-value

Synergism/0.22

Synergism/0.1

72 h

GCB

0.9 ± 0.18

0

14.3 ± 2.8

3.2 ± 4.5

TQ

64.9 ± 14.5

1.6 ± 1.3

165.1 ± 2.8

0. 1 ± 0.15

GCB + TQ

0.06 ± 0.01

0

2.3 ± 0.2

0

CI-value

Synergism/0.15

Synergism/0.25

  1. Data is presented as mean ± SD; n = 3.
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