Figure 5 | Scientific Reports

Figure 5

From: MicroRNA 375 modulates hyperglycemia-induced enteric glial cell apoptosis and Diabetes-induced gastrointestinal dysfunction by targeting Pdk1 and repressing PI3K/Akt pathway

Figure 5

Transfection of microRNA375 inhibitor in vitro. (A) AnnexinV- FITC/PI flow cytometry analysis of apoptosis of EGC transfected with microRNA375 inhibitor or microRNA375 inhibitor negative control. (B) Western blot analysis of Cleaved Caspase-3, Bcl-2, Pdk1 and p-Akt in EGC transfected with microRNA375 inhibitor or miRNA375 inhibitor negative control. 200 mM + control, EGC treated with 200 mM glucose concentration and transfected with microRNA375 negative control. 200 mM+ inhibitor, EGC treated with 200 mM glucose concentration and transfected with microRNA375 inhibitor. Full-length gels and blots are included in Supplementary materials. Result presented as mean ± SD. ***P < 0.001, apoptosis of EGC treated with 200 mM glucose compared to EGC treated with 25 mM glucose; ###P < 0.001, apoptosis of EGC treated with 200 mM glucose + microRNA375 inhibitor compared to EGC treated with 200 mM glucose.

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