Figure 6

Estimation of gdv1 and AP2G transcripts in 44 individual P. falciparum clinical infection samples from Ghana and Senegal as measured by RT-qPCR using primer pairs within each coding sequence. (A) Ratios (Log₁₀) of gdv1 to a housekeeping gene (serine tRNA ligase) show a wide spectrum that is similar in each population. (B) Ratios (Log₁₀) of AP2G to the housekeeping gene shows a similarly wide spectrum that does not differ between the populations. (C) Ratios (Log₂) of the two gametocyte commitment genes gdv1 / AP2G (these ratios were not determined for 7 of the clinical infections due to undetectable transcript measurement for either gene). These distributions are not significantly different between the two countries (Mann-Whitney P > 0.1). It should be noted that the assays do not discriminate between sense mRNA and antisense transcripts which have recently been described to be involved in regulation of gdv1⁵⁰.