Figure 1 | Scientific Reports

Figure 1

From: CRISPR/Cas9-mediated mutagenesis of homologous genes in Chinese kale

Figure 1

A map of the constructed vector. (A) Schematic of the BaPDSs gene fragment indicating the sgRNA target site and sequence. indicates exon. indicates intron. (B) Gel electrophoresis of the recombinant plasmid of pSG-BaPDS and pCC. M, DL15000 marker. (C) Structure of the CRISPR/Cas9 binary vectors for Chinese kale transformation. The Cas9 cassette was driven by the 35S promoter, while sgRNA was controlled by the hU6 promoter. The 3 × Flag is a label which can be used to identify the transgenic plants. NLS, nuclear localization sequence.

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