Figure 4
From: Transthyretin Aggregation Pathway toward the Formation of Distinct Cytotoxic Oligomers
(a) Experimental CD spectra of tetrameric WT TTR (black), dimer (red), and oligomer (green) eluting at 61 mL in Fig. 1, along with reconstructed dotted spectra using the software DichroWeb. The protein concentration of 0.2 mg/ml (monomer concentration) was used for the protein samples. (b) Overlaid two-dimensional (2D) 13C-13C correlation solid-state NMR spectra of WT (native) and WT oligomer (red) obtained with COmbined R2n(v)-Driven (CORD)58 recoupling scheme. (c) Overlaid 2D spectra of WT (black) and G53A (red) oligomers. The CORD mixing time was 115 ms.
