Figure 6
From: Erythrocytes as bioreactors to decrease excess ammonium concentration in blood
Model of hyperammoniemia in mice in vivo. (a) Decrease of ammonia in the blood of mice (Swiss line, male, weight 30 g) with hyperammonemia, which was induced by one injection into the tail vein of ammonium acetate at a dose of 2.5 mmol/kg. Each animal (n = 12) received 135 µl of ERBs, containing glutamate dehydrogenase and alanine aminotransferase (1.313 ± 0.106 and 1.612 ± 0.197 IU/mlRBCs for GDH and AAT, respectively) in normal saline (total volume 0.4 ml) (a curve EBRs + C). The final hematocrit of EBRs in the blood of mice was approximately 5.4%. As a control, animals were used, which received, instead of ammocytes, cells that passed all stages of the encapsulation procedure, but without introducing enzymes (n = 12) (curve Control). (b) Comparison of experimental curve of AMM decreasing in mice (1) with curves calculated using a mathematical models of EBRs: (2) – a model for human erythrocytes (concentrations of AKG and GLU were set as in human RBCs (0.005 and 0.5 mmol/lRBCs, respectively)); (3) – a mouse model with 0.005 and 0.1 mmol/lRBCs, for AKG and GLU, respectively; (4) – a mouse model with the increased AKG and GLU concentrations (0.15 mmol/lRBCs and 1.5 mmol/lRBCs, respectively).
