Figure 9
Lysosome disruption after PDT. T98G cells were incubated with TAZnPc (grey bars) or ZnPc (black bars) for 18 hours. Then, the media was discarded and cells incubated with Lysotracker for two hours to allow the accumulation of the probe in lysosomes. After the incubation, media was changed, and the cells irradiated with 10 or 27/cm2. One hour after irradiation total cells were collected and the Lysotracker Green fluorescence was assessed using flow cytometry. Quantification of the mean fluorescence intensity (MFI) is shown in the bar graph. Results are presented as mean ± SEM of MFI of a representative experiment made in triplicate. **p < 0.01, *p < 0.05 with respect to the cells without irradiation (0 J/cm2) using Two way ANOVA with Dunnett’s post test.
