Figure 3
From: De novo origins of multicellularity in response to predation
Median cluster sizes of evolved strains at the time-point where strains reached maximum size. To determine the time where strains were largest, we calculated the mean from five replicate medians (open dots), for each strain and time-point. Because data are not normally distributed, medians were chosen to approximate central tendency. Cells per cluster were measured by sampling strains over six days of growth, staining nuclei with DAPI, and imaging using fluorescent microscopy. From left to right, time-points of maximum size for each strain were: 12, 12, 72, 120, 108, 84, 96, 96, and 72 hours. From left to right, median sizes of replicate populations averaged 1, 1, 1.4, 1.8, 1.8, 4, 6.2, 5.6 and 5.6 cells per cluster. Letters above columns indicate significant differences among medians (Kruskal-Wallis, \({{\rm{{\rm X}}}}_{8}^{2}=38.49\), p < 0.001, Dunn’s Test for multiple comparisons, α = 0.05). Sizes at the initial time-point (0 hrs) were omitted from analysis because they represent starting conditions. Shading is only for ease of visualization.
