Table 3 Single editing site contribution to changes in editing patterns of different 5-Ht2cr pre-mRNA isoforms between PWScrp−/m5′LoxP and wild type mice choroid plexus samples.

From: Ectopic expression of Snord115 in choroid plexus interferes with editing but not splicing of 5-Ht2c receptor pre-mRNA in mice

Edited Site

Mean RPKM WT

Mean RPKM LoxP

Fold change (WT/LoxP)

P-value, SIG

WT, %

LoxP, %

1

2275

2225

1

0.67, no

0.2

0.2

2

4129

3295

1.3

0.14, no

0.4

0.4

3

2119

2078

1

0.8, no

0.2

0.2

A

148907

100311

1.5

0.000057****

14.7

11.3

B

66074

37479

1.8

0.0003***

6.5

4.2

C

234736

190987

1.2

0.0004***

23.2

21.6

D

904161

782103

1.2

0.006**

89.5

88.4

E

51944

45946

1.1

0.15, no

5.1

5.2

F

1877

1891

1

0.94, no

0.2

0.2

G

3242

2378

1.4

0.14, no

0.3

0.23

H

1940

1878

1

0.84, no

0.2

0.26

  1. Editing site: The editing sites are labeled according to Fig. 1A. Mean RPKM WT and LoxP: Calculated mean of all RPKM values for 5-Ht2cr mRNA editing patterns accounting for site contribution in wild type (WT) and, PWScrp−/m5′LoxP (LoxP) editing. Fold change WT/LoxP: Fold change between wild type (WT) and PWScrp−/m5′LoxP (LoxP) editing sites. P-value: P-value of Student’s t-test. SIG: Graphpad formatting - asterisks describe p-values levels of statistical significance. False Discovery Rate (pBH) values were calculated for the correction of multiple testing; pBH values 0.0006, 0.001, 0.0016 and 0.016 for editing sites A, B, C and D, respectively, confirmed significances of the t-test. WT(%) and LoxP(%): The percentage of detected editing in the depicted sites in wild type (WT) and PWScrp−/m5′LoxP (LoxP) mouse choroid plexuses is indicated. Percentage is calculated based on the mean RPKM values for 5-Ht2cr mRNA-edited isoforms in investigated genotypes.
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