Figure 6

Gene expression in Clock^∆19/∆19 versus WT mPFC and hippocampus, and adaptations in response to HF. (a) Gene expression was assessed in Clock^Δ19/Δ19 and WT mPFC and hippocampus at baseline (BL), after MI, and in HF, by using microarrays and bioinformatics analyses. (b) Key Gene Ontology (GO) pathways identified for genes with ≥ 1.3-fold difference in Clock^Δ19/Δ19 versus WT mice, in the mPFC (dark boxes) or hippocampus (light boxes). (c) Baseline conditions. Circadian mechanism and output genes that differ in expression (≥ 1.3-fold change) in the mPFC (top) or hippocampus (bottom) for Clock^Δ19/Δ19 versus WT mice. (d) Response to MI. Genes that differ in expression (≥ 1.3-fold change) in the mPFC of Clock^Δ19/Δ19 versus WT mice. (e) Response to HF. Clock^Δ19/Δ19 versus WT genes that exhibit dichotomous expression at baseline, and then converge in HF, mPFC (top) or hippocampus (bottom). For images in Fig. 6, RFU  =  relative fluorescence units, *P < 0.05 by Student’s t-test, further information on genes shown is provided in Table 2, and all gene shown as well as additional identified genes are further detailed in Supplementary Table S1.