Figure 3

Time kinetics for EBOV/Mak inactivation efficacy results for the 1:20 dilution of DAL lots 15083E, 16004E, and 16005E at ambient temperature. The values represent the mean ± standard deviation (n = three replicates, one for each DAL lot) of the log₁₀ titer of the positive control (0 minutes contact time) and the post-neutralization samples (0.5, 1, 5, and 10 minutes contact time). Individual viral titers were calculated based on three replicate wells per dilution. The limit of detection (LOD) of the assay was 1.8 log₁₀ TCID₅₀/mL (shown in the plot as a blue line extending from y = 1.8 log₁₀ TCID₅₀/mL). This was due to the cytotoxicity of the DAL dilution to the Vero E6 cells.