Figure 4 | Scientific Reports

Figure 4

From: A sensitive and simple targeted proteomics approach to quantify transcription factor and membrane proteins of the unfolded protein response pathway in glioblastoma cells

Figure 4

Quantification of UPR proteins in different glioblastoma cell lines non-treated and treated with Bortezomib using Orbitrap Fusion Lumos Tribrid Mass Spectrometer. Applying the optimized PRM assay, the expression level of the low abundancy UPR proteins at basal level and under treatment with 1 nM Bortezomib could be determined. The ratios between internal standard peptide and the endogenous peptide (light to heavy ratio) are presented. All the measurements were performed with three biological replicates.

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