Figure 3 | Scientific Reports

Figure 3

From: Quantitative Profiling of Oncometabolites in Frozen and Formalin-Fixed Paraffin-Embedded Tissue Specimens by Liquid Chromatography Coupled with Tandem Mass Spectrometry

Figure 3

Chiral derivatization followed by LC-MS/MS analysis for determination of D- and L-enantiomers of 2HG. (A) Schematic illustration of derivatization of D- and L-2HG by N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) followed by chromatographic separation of D- and L-2HG TSPC derivatives. (B) MS/MS spectra of the TSPC derivatives of D- and L-2HG as well as isotope-labeled D- and L-2HG. (C) Extracted chromatograms of TSPC derivatives of D- and L-2HG as well as their respective isotope-labeled internal standards in the blank (LC-MS grade water), spiked at the LLOQs in water, and the extract from pooled homogenate of FFPE or frozen tissue.

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