Figure 4

Flow cytometric analysis of SVF cells obtained from human SAT and VAT. Representative flow cytometric dot plots of surface markers CD34 vs CD31 determining the percentage of adipose stem cells (ASCs) (CD45−/31−/34+), endothelial progenitor cells (CD45/31+/34+) and endothelial mature cells (CD45−/31+/34−) within SVF freshly isolated from SAT and VAT of the lean control subjects and normoglycemic (ob N), prediabetic (ob preDM) and diabetic (ob T2DM) obese patients (A). Quantification of ASCs (B,C), endothelial progenitor (D,E) and endothelial mature cells (F,G) contained in SVF from SAT (white boxes, B,D,F) and VAT (grey boxes, C,E,G). The percentage of cells were displayed as box plot graphs with the lowest and highest values (whiskers), the medians (lines), the means (dashed lines) and the outliers (black circles). The number of AT samples analyzed for each group was: 4 lean, 20 ob N, 15 ob preDM, 23 ob T2DM in SAT and 9 lean, 23 ob N, 21 ob preDM, 30 ob T2DM in VAT. The data was analyzed using the One Way ANOVA test followed, when statistically significant, by Fisher LSD test in (B–D) and Kruskal-Wallis test (Dunn’s method) in (E–G), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.