Table 2 Organisms used in this study.
From: The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence
Bacteria/Nematodes/Arthropods/Annelids | Genotype, relevant characteristics and usesa | Reference or source |
|---|---|---|
E. coli MC4100 | F- araDI39 Δ(argF-lac) U169 rpsL150 relAl flb5301 deoCI ptsF24 rbsR. Used to evaluate the presence of antibiotic compounds in supernatants of PAO1 strains. | |
E. coli OP50 | Uracil auxotroph. Used to feed C. elegans. | |
E. coli β2163 | KmREmR; F− RP4-2-Tc::Mu ΔdapA::(erm-pir). | |
E. coli DH5α | supE44 lacU169 (Ø80lacZΔ M15) hsdR17 (rk-mk-) recA1 endA1 gyrA96 thi-1 relA1. | |
B. cepacia CC-Al74 | Wild type. Risk Group 2 bacteria and proposed as plant growth promoting rhizobacteria (PGPR). Used as a pathogenic control in virulence assays. | |
P. putida KT2440 | Wild type; Risk Group 1 bacteria and PGPR. Used as negative control in virulence assays. | |
V. fischeri ATCC 49387 | Wild type; Used as bioluminescent strain in MicroTox® assays. | |
P. aeruginosa PAO1 | Wild type pathogenic strain. | |
P. aeruginosa ΔmcpB | In-frame deletion mutant in mcpB (1116 bp Δ). | This study |
C. elegans Bristol strain N2 | Nematode used in virulence assays. Provided by the Laboratory of Nematology, National Museum of Nature Sciences-CSIC (Madrid, Spain) | Navas et al., 2007 |
A. bipunctata | Arthropod used in virulence assays. Supplied by ControlBio Co. (Almería, Spain). | Ref. CBi K04884 |
C. carnea | Arthropod used in virulence assays. Supplied by ControlBio Co. (Almería, Spain). | Ref. CBi 124 K04280 |
E. foetida | Annelid used in virulence assays. Supplied by Lombriventa (Gerona, Spain). | |
D. magna | Crustacean used in virulence assays. Supplied by Daphtoxkit™ (Creasel, Belgium). |
