Figure 3
From: Semaphorin 6A Attenuates the Migration Capability of Lung Cancer Cells via the NRF2/HMOX1 Axis
Expression of HMOX1 and migration capability by transwell assay of H1299 cells transfected with different vectors. (A) Relative mRNA expressions of HMOX1 in HMOX1 -overexpressing (HMOX1 O/E) (left) or -silenced (shHMOX1) (right) H1299 cells. (B) Migration capability of H1299 cells overexpressing HMOX1 or empty vector (pCDH). Inset: western blot of HMOX1. (C) Migration capability of HMOX1-silenced H1299 cells and cells containing empty vector (pLKO.1). Inset: western blot of HMOX1. (D) mRNA and (E) protein expression of HMOX1 in H1299 cells co-transfected with either pLKO.1 + pCDH, pLKO.1 + 6A-FL, shHMOX1 + pCDH, or shHMOX1 + 6A-FL. (F) Migration capability of H1299 cells co-transfected with either pLKO.1 + pCDH, pLKO.1 + 6A-FL, shHMOX1 + pCDH, or shHMOX1 + 6A-FL. *Statistical significance compared to empty vector-transfected H1299 cells at p < 0.05, n = 3. # Statistical significance compared to each other at p < 0.05, n = 3.
