Table 2 Thermodynamic and kinetic characterization.

From: Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition

Lipase

Transition rates [s−1]

k1

k−1

k2

k−2

k3

k−3

L2

2.43 ± 0.014

1.38 ± 0.010

1.59 ± 0.007

2.33 ± 0.011

  

L3

1.30 ± 0.023

1.16 ± 0.013

  

1.67 ± 0.006

5.64 ± 0.017

Native

0.95 ± 0.040

1.97 ± 0.095

  

2.89 ± 0.041

7.72 ± 0.082

Native product

1.00 ± 0.032

0.72 ± 0.039

0.79 ± 0.023

0.85 ± 0.037

  

L3 product

0.97 ± 0.12

1.00 ± 0.10

1.46 ± 0.071

1.34 ± 0.06

  
  1. *Error corresponds to one standard deviation.
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