Figure 2 | Scientific Reports

Figure 2

From: Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies

Figure 2

GeNorm stability analysis of Bd candidate reference genes. The stability of the genes was assessed during different growth stages with (a,e,i) a combination of all growth stages (D0 + D3 + D5) (n = 18), (b,f,j) fresh spores at day 0 (D0) (n = 6), (c,g,k) sporangia at day 3 (D3) (n = 6) and (d,h,l) a mature culture at day 5 (D5) (n = 6). Genes were ranked based on (a–d) the GeNorm M and (e–h) coefficient of variation (CV) value. (i–l) By pairwise variation (V) analysis, the optimal number of reference genes was determined.

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