Figure 5
From: DNA methylation of a NF-κB binding site in the aquaporin 5 promoter impacts on mortality in sepsis
Electrophoretic Mobility Shift Assay (EMSA) was performed with oligonucleotides representing the promoter region nt-922 to nt-940, including the CpG site at nt-937. Representative blot. The addition of nuclear extracts to labelled oligonucleotides resulted in the formation of two bands (lane 2). Addition of an excess of non-labelled oligonucleotide outcompeted the specific bands (lane 3). The upper specific band is diminished with the addition of NF-κB antibody (lane 4).
