Figure 1
Demetallation of Cu•HSA by EDTA monitored by LC-ICP MS. Conditions: 10 μM Cu•HSA, 20 μM EDTA; incubation buffer 50 mM HEPES, 50 mM NaCl, pH 7.4; incubation times: (a) - 0 min, (b) - 1 min, (c) −10 min, (d) - 30 min, (e) - 60 min, (f) - 90 min, (g) - 160 min; column - 1 ml Sephadex G25 Superfine; elution buffer 200 mM NH4NO3, pH 7.4; flow rate 0.4 ml/min; injection volume 10 μl. Cu-63 was monitored by ICP MS. Figure was created by program Origin 9 Pro (https://www.originlab.com/).
