Table 1 RT-PCR primer list for MEIS target and CDKI genes.
From: Development of Small Molecule MEIS Inhibitors that modulate HSC activity
Gene | Forward Primer | Reverse Primer |
|---|---|---|
GAPDH | TTGATGGCAACAATCTCCAC | CGTCCCGTAGACAAAATGGT |
Meis1 | GTTGTCCAAGCCATCACCTT | ATCCACTCGTTCAGGAGGAA |
P15 (CDK2NB) | CAGTTGGGTTCTGCTCCGT | AGATCCCAACGCCCTGAAC |
P16 (CDKN2A) | GGGTTTCGCCCAACGCCCCGA | TGCAGCACCACCAGCGTGTCC |
P18 (CDK2NC) | CTCCGGATTTCCAAGTTTCA | GGGGGACCTAGAGCAACTTAC |
P19 (CDKN2D)- | TCAGGAGCTCCAAAGCAACT | TTCTTCATCGGGAGCTGGT |
P19-ARF | GTTTTCTTGGTGAAGTTCGTGC | TCATCACCTGGTCCAGGATTC |
P21 (CDKN1A) | ATCACCAGGATTGGACATGG | CGGTGTCAGAGTCTAGGGGA |
P27 (CDKN1B) | GGGGAACCGTCTGAAACATT | AGTGTCCAGGGATGAGGAAG |
P57 (CDKN1C) | TTCTCCTGCGCAGTTCTCTT | CTGAAGGACCAGCCTCTCTC |
Hif-1α | CGGCGAGAACGAGAAGAA | AAACTTCAGACTCTTTGCTTCG |
Hif-2α (EPAS1) | ATCACGGGATTTCTCCTTCC | GGTTAAGGAACCCAGGTGCT |
Hif-3α | TGTGAACTTCATGTCCAGGC | GCAATGCCTGGTGCTTATCT |
