Fig. 5: Validation of transcriptomic data. | Communications Biology

Fig. 5: Validation of transcriptomic data.

From: In vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae

Fig. 5

Gene expression values from the dual RNA-seq were confirmed by qRT-PCR, using a the same isolated RNA used for the dual RNA-seq or b isolated RNA from a repeated experiment. a 18 murine and 19 pneumococcal genes were chosen as experimental validation targets. Log2 fold changes were plotted from qRT-PCR against dual RNA-seq log fold changes for 9–47-Ear versus 4559-Blood, 9–47-Ear versus 9–47M, 9–47M versus 4559M, and 4559-Blood versus 4559M comparisons. A total of 72 murine and 76 pneumococcal comparisons were plotted, with a high degree of correlation observed for both species (R2 > 0.73, Pearson). b 6 murine and 6 pneumococcal genes were chosen as targets to test the reproducibility of the dual RNA-seq data. Log2 fold changes were plotted from qRT-PCR against dual RNA-seq log fold changes for 9–47-Ear versus 4559-Blood, 9–47-Ear versus 9–47M, 9–47M versus 4559M and 4559-Blood versus 4559M comparisons. A total of 24 murine and 24 pneumococcal comparisons were plotted, with a high degree of correlation observed for both species (R2 > 0.73, Pearson).

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