Table 2 Confirmation of essential genes in S. pneumoniae and S. mutans.

From: Genome-wide essential gene identification in Streptococcus sanguinis

SP_ID*

Product Name

CFU †

Gel ‡

Essential

SMU_ID*

CFU

Gel ‡

Essential

SP_0382

diphosphomevalonate decarboxylase

0

 

yes

SMU.937

0

 

yes

SP_0383

phosphomevalonate kinase

0

 

yes

SMU.938

0

 

yes

SP_0384

Isopentenyl pyrophosphate isomerase

0

 

yes

SMU.939

0

 

yes

SP_1514

F0F1 ATP synthase subunit C

2

2-band

yes

SMU.1534

1

2-band

yes

SP_1513

F0F1 ATP synthase subunit A

2

2-band

yes

SMU.1533

0

 

yes

SP_1511

F0F1 ATP synthase subunit delta

6

2-band

yes

SMU.1531

1

2-band

yes

SP_0262

phosphatidate cytidylyltransferase

0

 

yes

SMU.1785

0

 

yes

SP_0261

undecaprenyl pyrophosphate synthase

0

 

yes

SMU.1786

1

2-band

yes

SP_0489

PAP2 family protein

242

1-band

no

SMU.1702c

874

1-band

no

  1. *Gene identifiers. IDs in the same row indicate orthologous genes
  2. †Number of transformants obtained from attempt to mutagenize the gene indicated
  3. ‡Appearance of PCR products resulting from amplification of the target gene locus from selected transformants
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