Figure 5

Box and whisker plots depicting population distribution of percentage corrected FRET and showing maximum, minimum, upper & lower quartiles and sample median.

Bleach regions tested were inside nuclear foci (foci, f) and outside nuclear foci (non-foci, n). (A) CFP-U2AF65 Vs YFP-Ataxin-1 (wild-type, Alanine and Aspartic acid substitution mutants). B) CFP-Wt-14-3-3 (orange) and CFP-14-3-3- NLS (blue) Vs YFP-Ataxin-1 (wild-type, Alanine and Aspartic acid substitution mutants). Means +/− standard errors, rounded to one decimal place, are shown above each boxplot. Statistical significance bars are shown and represent results of unpaired t-tests of mean difference = 0 (* = P<0.05,** = P<0.01, *** = P<0.001). N numbers (left to right) are: (A) N = 16, N = 10, N = 24, N = 22, N = 21, N = 21; (B) N = 15, N = 12, N = 22, N = 26, N = 22, N = 14, N = 19, N = 15; and represent number of individual bleach events pooled from at least 4 individual cells.