Abstract
Cyclodextrins (CDs) can hardly self-assemble into well-defined structures. Here we report if they preassemble into channel type dimers assisted by dodecane, well-defined vesicles and bricks can be formed. Unlike the traditional self-assembly of amphiphilic molecules driven by hydrophobic effect, the self-assembly of the channel type dodecane@2β-CD supramolecular building block is predoninantly driven by hydrogen-bonds. More water molecules are found in the lyophilized vesicles than in the bricks, suggesting water molecules play an important role in the self-assembly of the channel-type dimers of β-CD. The amount of structural water in the self-assembly is closely related to the curvature of the final self-assembled structures. Our work reveals that the channel-type dimer of β-CD may represent a new sort of building block for advanced structures.
Similar content being viewed by others
Introduction
Molecular self-assembly has been verified a powerful protocol to fabricate a rich variety of nanostructures1,2,3. In most cases, hydrophobic effect occurs in self-assembling systems, such as the self-assembly of surfactants, membrane lipids and other amphiphiles3,4. Recently, however, the nonamphiphilic self-assemblies, especially vesicles, are attracting considerable attention owing to their contribution to the fundamental science, especially to the understanding of the driving forces for molecular self-assembly5,6,7. It has been found that the nonamphiphilic vesicles can be driven by electrostatic interactions, dipole interactions and van der Waals interactions, etc. A typical example is the self-assembly of polyoxometalate macroions5, where van der waals interaction and electrostatic interaction are expected to be the main driving forces for the vesicle formation. However, so far the mechanism for various nonamphiphilic vesicles' formation is still unclear. Large amount of case-dependent knowledge is required before the mystery is completely solved.
Herein we report a case of nonamphiphilic vesicle which is driven predominantly by hydrogen bond in the dodecane/β-cyclodextrin host-guest inclusion system. Cyclodextrins (CDs) are doughnut ring-like structures with a hydrophilic exterior and a hydrophobic cavity8. The hydrophobic cavity forms an ideal harbor in which poorly water-soluble molecules can shelter their most hydrophobic parts9. Therefore, CDs are able to form host-guest complexes with many molecules which change their self-assembling properties10,11,12,13. For instance, Jiang et al reported the formation of amphiphiles upon addition of CDs into a hydrophobic compound14. In most cases, the host-guest complexes based on CDs either self-assemble via hydrophobic interactions10,11 or lose the self-assembling abilities due to the increased solubility in water12,13.
However, our study revealed that the dodecane/β-CD inclusion system may self-assemble predominantly through hydrogen bonding into vesicles. Dodecane and β-CD was found to form 1:2 supramolecular complex dodecane@2β-CD exclusively. Because the extending length of dodecane is much shorter than the sum of two β-CDs, the dodecane molecule was completely buried in the channel formed by two β-CD molecules. Thus, dodecane was not directly involved in the interactions between the dodecane@2β-CD supramolecular complexes, so that the self-assembly of dodecane@2β-CD is simply that of the channel type β-CD dimers which is dominated by hydrogen bonds.
Hydrogen bonds occur very often in self-assembled one dimensional or network structures15,16,17. They also happen together with other weak interactions, such as hydrophobic effect and coordination, in various molecular self-assemblies18,19,20, but have never been reported being the major driving forces for the formation of vesicles. In this regard, the channel type β-CD dimer induced by dodecane (dodecane@2β-CD) has represented a new class of supramolecular building block that leads to nonamphiphilic vesicles. This is in clear contrast with the well-known poor self-assembling behaviors of various CD monomers. It is expected that besides dodecane, other alkanes and compounds can also induce various channel type CD oligomers, such as dimer, trimer or tetramer, etc. These oligomers as powerful building blocks may further self-assemble into various advanced structures. We believe this result not only highlights a crucial feature of the supramolecular chemistry of CDs, but also sheds lights on the physics of self-assembling behavior. Meanwhile, it may open a new vista for material science.
Results
Exclusive formation of 1:2 inclusion complexes between dodecane and β-CD
Figure 1a shows the macroscopic phase behaviour of β-CD solutions before and after addition of dodecane. The solution of β-CD is transparent at the concentration of 16 mM, whereas white precipitate was created upon the addition of 8 mM dodecane, indicating the formation of inclusion complexes between β-CD and dodecane21,22. The quantity of precipitates was almost doubled as the amount of dodecane was increased to 16 mM. However, mass analysis with 1H NMR23,24,25 revealed that the ratios between β-CD and dodecane in both precipitates were nearly 2:1 (see Supplementary Fig. 1). This means that the inclusion complex was predominantly dodecane@2β-CD which is independent of the initial material ratio. This is in clear contrast with the inclusion complex formed between β-CD and surfactant, where the resulting complex is mixing ratio dependent18,26. On the other hand, ESI-MS measurement showed the presence of supramolecular species [dodecane@2β-CD+CH3OH+3H]3+ (m/z 824.89503, the theoretical value m/z 824.99867) (Fig. 1b), which further confirmed the formation of dodecane@2β-CD. One may notice that the difference between the theoretical calculation and experimental result is much larger than the usual errors with a magnitude of several ppm in MS measurements. This larger error in our case is attributed to the following two reasons: 1) the large molecular mass for the [dodecane@2β-CD+CH3OH+3H]3+ supramolecular species exceeds the upper limit of 2000 for the HT-ESI-MS; 2) the charge neutral nature of the original dodecane@2β-CD supramolecular structures makes it difficult to obtain signals of charged molecular species so that the relative intensity has no direct relation with the content of the representing species27. As to the presence of CH3OH, it is probable aroused from the hydrogen bonding between β-CD and CH3OH, since the measurements were made in the methanol atmosphere.
The structure of dodecane@2β-CD verified with Small Angle X-ray Scattering (SAXS)
The formation of the 2:1 inclusion complex suggested that two β-CDs have been threaded with one dedecane chain to form channel type dimers, as demonstrated in Fig. 2a. This is further confirmed by the SAXS measurements. It is well-known that two possible crystalline structures, namely, cage-type and channel-type28, can be formed through β-CD/guest inclusion complexes. The cage-type structures display characteristic peaks at 6.8, 9.1, 9.4 and 12.8 nm−1, while the channel-type ones exhibit characteristic peaks at 8.2 and 12.4 nm−1 in their XRD patterns18. In Fig. 2b, we show the SAXS pattern of the lyophilized white precipitates, which is dramatically different from that of cage-type β-CD powders, but are in perfect agreement with that of channel type ones.
The self-assembled structures based on the supramolecular dodecane@2β-CD complex
The regular scattering patterns indicate the formation of well-organized microstructures. SEM observation of the precipitates (Fig. 3a) revealed the formation of ‘brick’-like structures. Large area of observation with light microscopy (Fig. 3b) also confirms the formation of polydispersed bricks from the basic supramolecular building block of dedecane@2β-CD. Moreover, it is interesting to find that vesicles were formed at low concentrations where precipitation was avoided. Figure 3c depicts the TEM image of the freeze-fractured sample (FF-TEM) for the 1 mM sample which is diluted from the precipitated one in Fig. 1a. Vesicles with diameters of 50 ~ 200 nm were observed. Angle-dependent DLS measurements also revealed the presence of spherical structures with average radius of 100 nm (see Fig. 3d). The hollow nature of these spherical particles was verified with AFM observations (Fig. 4 a–c). It is known that the extending length for dodecane and the height of β-CD is about 1.2 and 0.8 nm, respectively. This means that the dodecane should be completely buried in the hydrophobic channels formed by two β-CDs. Therefore, the thickness of the single layer of vesicle membrane under AFM should be around 1.6 nm. For a dried unilamellar vesicle, the height of 3.2 nm is expected. This was indeed the case. First of all, the size for the spherical particles in Fig. 4a was consistent with that obtained in Fig. 3c and 3d. Most importantly, the sectional height profile in Fig. 4b for two spheres were found being ~3.2 nm and 1.6 nm, respectively. The 3.2 nm thickness is obviously resulted from a collapsed unilamellar vesicle, while the one of 1.6 nm can be attributed to the free channel type β-CD dimers which didn't participate into self-assemble or be aroused from part of the broken membrane. Figure 4c shows there were also sectional height about 6.4 nm and 4.8 nm, which corresponds to a collapsed and a broken double lamellar vesicle, respectively.
Discussion
The self-assembling behavior of dodecane@2β-CD supramolecular inclusion complex is fairly amazing since most self-assembling systems require contradictory properties inside one building block. Typical examples are the amphiphilic surfactant and polymers. However, no hydrophobic portion can be found for the supramolecular building block of dodecane@2β-CD since the 1.2 nm dodecane is completely buried in the 1.6 nm channel formed by two β-CDs. This means that the self-assembly of the dodecane@2β-CD is a matter of self-assembly of the channel type dimer of β-CD. Then the driving force for the self-assembly formation is almost the same as that for the crystalline behavior of β-CD unimer. Namely, hydrogen bonding is crucial for the self-assembly of the dodecane@2β-CD supramolecular building blocks29,30,31. To verify this point, urea, which is famous for its role in breaking hydrogen bonds, was added to the 16 mM system where precipitates exist. It was observed that the white precipitates disappeared immediately in the presence of 5 M urea, (see Supplementary Figure S2), indicative of the disassembly of the bricks and verifying the importance of hydrogen bonds in keeping the self-assembles structures.
In regard to the formation of vesicles at low but bricks at high concentrations, it is possible that the different content of water in the self-assembled structures has resulted in different curvatures. At low concentrations, more water molecules are expected to bridge the dodecane@2β-CD supramolecular building blocks. The uneven distribution of water molecules in the outer and inner side of the membrane leads to the curvature for vesicles. In contrast, at high enough concentrations, there are enough β-CD dimers in the system so that direct hydrogen bonding between the hydroxyl groups of neighboring β-CD is available. The parallel packing of the β-CD dimers allows formation of planar self-assemblies, which is brick in this case. As shown in Fig. 5, the wave number for the hydroxyl group in the ‘bricks’ shifts to a much lower value compared with that in the vesicles, which demonstrates that the hydrogen bond in the bricks is stronger than that in vesicles32. This means that the content of the hydrogen bonds between H2O and the –OH of β-CD in the vesicular system is much more than that in the ‘bricks’33. Meanwhile, the DSC and TGA measurements also demonstrated that the structure water have been lowered in the bricks (Fig. 6). The DSC curve for the lyophilized vesicles exhibits an endothermic peak centered at 45°C. Upon slowing down the scanning rate from 2.0 K/min to 0.5 K/min, DSC did not resolve the endothermic peak into separate events, indicating that all the water molecules are simultaneously eliminated. On the other hand, TGA analysis for the lyophilized vesicles shows that the weight losses of water are 13.2% (see Fig. 6a). This weight loss is attributed to the structural water of the vesicles34. That's to say, averagely each dodecane@2β-CD supramolecular building block binds about 21 water molecules in order to form vesicles [(13.2/18.0)/(86.8/2440.3) = 20.6]. In contrast, the DSC curve for the lyophilized bricks has two endothermic peaks at 50°C and 80°C (Fig. 6b), indicating the presence of water molecules in different environments. TGA results show that the weight losses corresponding to these two peaks are 6.5% and 1.5%, respectively. That's to say, the structural water in the bricks is only 8%, which is much less than the content of water in vesicles, which is in good agreement with the deduction based on the FT-IR spectra in Fig. 5. It should be also noticed in Fig. 5 that both wave numbers for the –OH groups in the vesicles and the bricks are lower than that for the powder of β-CD, indicating more water molecules have involved in the self-assembly of the β-CD dimers. It is possible the participation of water slows down the crystalline process so that well-defined structures are formed. In this point of view, the channel-type β-CD dimers can be viewed as a new class of building block which is different from the β-CD monomers. The whole process of self-assembly formation in the dodecane-β-CD system is illustrated in Fig. 7.
In conclusion, vesicles have been constructed with the channel type dimers of β-CD which is facilitated by dodecane. The addition of dodecane into the solution of β-CD allows the formation of dodecane@2β-CD supramolecular building blocks where the dodecane was encaged into the cavity of the channel formed by two β-CDs. The channel type β-CD dimers then self-assemble mainly via hydrogen bonding. At low concentrations, more water molecules were involved in the hydrogen bonding and the uneven distribution of the water molecules in the outer and inner side of the membrane provided the curvature for vesicle formation. At high concentrations, the amount of water participated in the hydrogen bonding is much less so that no curvature can be formed thus plannar structures are formed. We expect that besides dodecane, other alkanes and compounds can also induce various channel type CD oligomers, such as dimer, trimer or tetramer, etc. These oligomers as powerful building blocks may further self-assemble into various advanced structures.
Methods
Materials
n-Dodecane (99%) were purchased from Alfa Aesar. β-Cyclodextrin (β-CD) with a water content of 14% were purchased from Sinopharm Chemical Reagent Co. Ultrapure water was used throughout the work.
Sample preparation
For the precipitate samples, dodecane was added into the β-CD solutions of 16 mM at the initial host-guest stoichiometries of 2:1 and 2:2, respectively and then the samples were vortex mixed sufficiently to create the white precipitate. After removing the supernatant solution, the dodecane/β-CD precipitates from the initial host-guest stoichiometries of 2:1 and 2:2 were lyophilized to obtain white powders. For the vesicle sample, desired amounts of lyophilized dodecane/β-CD precipitates and water were weighed into test tubes to give the dodecane@2β-CD concentration of 1 mM. The resultant solutions were thermostatically incubated at 25°C (for at least 24 h) to allow self-assembly formation.
1H NMR measurement
The lyophilized precipitates or vesicles were dissolved into DMSO for 1H NMR measurements to determine the actual ratio of dodecane to β-CD. The measurements were performed on an AVANCE III 500 M Hz NMR (Bruker, Switzerland). All the proton signals were calibrated with the TMS at 0.00 ppm. By comparing the integration between β-CD (H1 protons, δ = 4.83 ppm) and dodecane (CH3 protons, δ = 0.83 ppm), dodecance/β-CD complex ratio can be determined.
Optical microscope observation
The dodecane/β-CD precipitates were dropped on the slide and observed with an optical microscope (Olympus BX51TRF with DP72-SET digital camera) equipped with polarizers in reflection mode under white-light illumination.
SEM observation
The dodecane/β-CD precipitates was mounted onto silicon wafer and air-dried before applied to SEM observation (SEM, Hitachi S4800, 5 kV).
SAXS measurements
The SAXS measurement of the β-CD power and lyophilized dodecane/β-CD precipitates was conducted on a high-flux small-angle X-ray scattering instrument (SAXSess, Anton Paar) equipped with a Kratky blockcollimation system. A Philips PW3830 sealed-tube X-ray generator (Cu Kα) was employed to simultaneously measure the small-angle and wide-angle X-ray scattering (WAXS and SAXS) of the samples. The scanning patterns extend to the high-angle range (the q range covered by the imaging plate is from 0.0 to 27.2 nm−1, q = 4πsinθ/λ, where λ is the wavelength of 0.1542 nm and 2θ is the scattering angle). The scattering angles were calibrated with aluminum.
ESI-MS measurements
FT-MS measurements were carried out on an APEX IV FT-MS (Bruker, USA). The operating conditions of the ESI source: positive ion mode; spray voltage −3300 V; capillary voltage −3800 V, capillary temperature 230°C; skimmer1 33.0 V, skimmer2 28.0 V; sheath gas nitrogen pressure 0.3 bar. The lyophilized precipitates were dissolved in pure methanol with the concentration of 0.1 mM and introduced via direct infusion at a flow rate of 3.00 μL/min.
Dynamic light scattering (DLS)
Dynamic light scattering (DLS) measurements were conducted on an ALV/DLS/SLS5022F light scattering-apparatus, equipped with a 22 mW He-Ne laser (632.8 nm wavelength) with a refractive index matching bath of filtered toluene surrounding the cylindrical scattering cell. The samples were filtered by 450 nm filters. The scattering angle was set from 30° to 90°.
Freeze-fracture transmission electron microscopy (FF-TEM)
A small amount of sample was placed on a 0.1 mm thick copper disk and then covered with a second copper disk. The sample was frozen by plunging into liquid propane cooled by liquid nitrogen. Fracturing and replication were performed on a freeze-fracture apparatus (BalzersBAF400, Germany) at-140°C. Pt/C was deposited at an angle of 45° to shadow the replicas and C was deposited at an angle of 90° to consolidate the replicas. The resulting replicas were observed in a JEM-100CX II electron microscope (JEOL, Japan).
Atomic force microscopy (AFM)
AFM measurements in tapping mode under ambient conditions were conducted on a D3100 AFM (VEECO, USA). One drop of the dodecane/β-CD solution was spin-coated on a mica surface and then placed at room temperature to dry before AFM observation.
Fourier Transform Infrared (FT-IR)
FT-IR measurements were performed on Nicolet Magna IR 750 equipped with an infrared microspectrography (Thermo Scientific Co., USA). The samples were frozen in liquid nitrogen and subsequently lyophilized for 48 h before FT-IR measurements.
TGA and DSC measurements
TGA and DSC measurements were conducted synchronously on a Q600SDT (Thermal Analysis Company, USA). The vesicle sample and brick sample were frozen in a liquid nitrogen bath followed by a vacuum freeze drying step before TGA and DSC measurements. The lyophilized samples were placed into the alumina crucible with air as the reference. The heating-rate was 2K/min from 25 to 120°C.
References
Whitesides, G. M. & Grzybowski, B. Self-assembly at all scales. Science 295, 2418–2421 (2002).
Coleman, A. C. et al. Light-induced disassembly of self-assembled vesicle-capped nanotubes observed in real time. Nat. nanotechnol. 6, 547–552 (2011).
Whitesides, G. M., Mathias, J. P. & Seto, C. T. Molecular self-assembly and nanochemistry: a chemical strategy for the synthesis of nanostructures. Science 254, 1312–1319 (1991).
Southall, N. T., Dill, K. A. & Haymet, A. A view of the hydrophobic effect. J. Phys. Chem. B 106, 521–533 (2002).
Liu, T., Diemann, E., Li, H., Dress, A. W. & Müller, A. Self-assembly in aqueous solution of wheel-shaped Mo154 oxide clusters into vesicles. Nature 426, 59–62 (2003).
Li, D., Zhang, J., Landskron, K. & Liu, T. Spontaneous self-assembly of metal-organic cationic nanocages to form monodisperse hollow vesicles in dilute solutions. J. Am. Chem. Soc. 130, 4226–4227 (2008).
Wu, L., Lal, J., Simon, K. A., Burton, E. A. & Luk, Y.-Y. Nonamphiphilic assembly in water: polymorphic nature, thread structure and thermodynamic incompatibility. J. Am. Chem. Soc. 131, 7430–7443 (2009).
Szejtli, J. Introduction and general overview of cyclodextrin chemistry. Chem. Rev. 98, 1743–1754 (1998).
Junquera, E., Tardajos, G. & Aicart, E. Effect of the presence of. beta.-cyclodextrin on the micellization process of sodium dodecyl sulfate or sodium perfluorooctanoate in water. Langmuir 9, 1213–1219 (1993).
Bojinova, T. et al. Complexes between β-cyclodextrin and aliphatic guests as new noncovalent amphiphiles: formation and physicochemical studies. Langmuir 19, 5233–5239 (2003).
Jing, B. et al. Self-Assembly Vesicles Made from a Cyclodextrin Supramolecular Complex. Chem-Eur J 13, 9137–9142 (2007).
Saenger, W. & Müller-Fahrnow, A. Cyclodextrins increase surface tension and critical micelle concentrations of detergent solutions. Angew. Chem. Int. Ed. Engl. 27, 393–394 (1988).
Jiang, L., Yan, Y., Drechsler, M. & Huang, J. Enzyme-triggered model self-assembly in surfactant–cyclodextrin systems. Chem. Commun. 48, 7347–7349 (2012).
Zou, J., Tao, F. & Jiang, M. Optical switching of self-assembly and disassembly of noncovalently connected amphiphiles. Langmuir 23, 12791–12794 (2007).
Bakota, E. L., Sensoy, O., Ozgur, B., Sayar, M. & Hartgerink, J. D. Self-assembling multidomain peptide fibers with aromatic cores. Biomacromolecules 14, 1370–1378 (2013).
Jiang, L. et al. “Annular Ring” microtubes formed by SDS@ 2β-CD complexes in aqueous solution. Soft Matter 6, 1731–1736 (2010).
Fichman, G. & Gazit, E. Self-assembly of short peptides to form hydrogels: Design of building blocks, physical properties and technological applications. Acta biomaterialia 10, 1671–1682 (2014).
Jiang, L., Peng, Y., Yan, Y. & Huang, J. Aqueous self-assembly of SDS@ 2β-CD complexes: lamellae and vesicles. Soft Matter 7, 1726–1731 (2011).
Rest, C. et al. Self-Assembly and (Hydro) gelation Triggered by Cooperative π–π and Unconventional CH···X Hydrogen Bonding Interactions. Angew. Chem. Int. Ed. 53, 700–705 (2014).
Shen, Z., Wang, T. & Liu, M. H-bond and π–π stacking directed self-assembly of two-component supramolecular nanotubes: tuning length, diameter and wall thickness. Chem. Commun. 50, 2096–2099 (2014).
Sanemasa, I. et al. Preparation of Cyclodextrin Precipitates with Volatile Hydrocarbons in Aqueous Media and Determination of Hydrocarbon/Cyclodextrin Molar Rations in the Precipitates. Bull. Chem. Soc. Jpn. 66, 1424–1429 (1993).
Dou, Z. P., Xing, H. & Xiao, J. X. Hydrogenated and Fluorinated Host–Guest Surfactants: Complexes of Cyclodextrins with Alkanes and Fluoroalkyl-Grafted Alkanes. Chem-Eur J 17, 5373–5380 (2011).
Wilson, L. D. & Verrall, R. E. A 1H NMR study of cyclodextrin-hydrocarbon surfactant inclusion complexes in aqueous solutions. Can. J. Chem. 76, 25–34 (1998).
Freitag, M., Gundlach, L., Piotrowiak, P. & Galoppini, E. Fluorescence Enhancement of Di-p-tolyl Viologen by Complexation in Cucurbit [7] uril. J. Am. Chem. Soc. 134, 3358–3366 (2012).
Tao, W. et al. A linear-hyperbranched supramolecular amphiphile and its self-assembly into vesicles with great ductility. J. Am. Chem. Soc. 134, 762–764 (2011).
Jiang, L., Yan, Y. & Huang, J. Zwitterionic surfactant/cyclodextrin hydrogel: microtubes and multiple responses. Soft Matter 7, 10417–10423 (2011).
Guo, M. et al. Studies on the non-covalent complexes between oleanolic acid and cyclodextrins using electrospray ionization tandem mass spectrometry. J. Mass Spectrom. 38, 723–731 (2003).
Harada, A., Okada, M., Li, J. & Kamachi, M. Preparation and characterization of inclusion complexes of poly (propylene glycol) with cyclodextrins. Macromolecules 28, 8406–8411 (1995).
Saenger, W. & Steiner, T. Cyclodextrin inclusion complexes: host-guest interactions and hydrogen-bonding networks. Acta Crystallogr. Sect. A: Found. Crystallogr. 54, 798–805 (1998).
Zhou, C. et al. Self-Assembly of Nonionic Surfactant Tween 20@ 2β-CD Inclusion Complexes in Dilute Solution. Langmuir 29, 13175–13182 (2013).
Bas, G. L. & Tsoucaris, G. Two-dimensional hydrogen bond network in β-cyclodextrin complexes. Supramol. Chem. 4, 13–16 (1994).
Ke, D., Zhan, C., Li, A. D. & Yao, J. Morphological Transformation between Nanofibers and Vesicles in a Controllable Bipyridine–Tripeptide Self-Assembly. Angew. Chem. 123, 3799–3803 (2011).
Manas, E. S., Getahun, Z., Wright, W. W., DeGrado, W. F. & Vanderkooi, J. M. Infrared spectra of amide groups in α-helical proteins: Evidence for hydrogen bonding between helices and water. J. Am. Chem. Soc. 122, 9883–9890 (2000).
Cheruzel, L. E. et al. Structures and Solid-State Dynamics of One-Dimensional Water Chains Stabilized by Imidazole Channels. Angew. Chem. 115, 5610–5613 (2003).
Acknowledgements
This work was supported by the Natural Science Foundation of China (21173011, 21273013), the Foundation for Innovative Research Group of the Natural Science Foundation of China (51121091) and the National Basic Research Program of China (973 Program, 2013CB933800).
Author information
Authors and Affiliations
Contributions
C.C.Z. performed all the experiments and involved in writing; X.H.C. and Q.Z. provided useful help in ESI-MS and FF-TEM experiments; Y.Y. proposed the study and revised the manuscript; J.B.H. and J.D.W. participated useful discussions. All authors reviewed the manuscript.
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Electronic supplementary material
Supplementary Information
Self-Assembly of Channel Type β-CD Dimers Induced by Dodecane
Rights and permissions
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
About this article
Cite this article
Zhou, C., Cheng, X., Zhao, Q. et al. Self-Assembly of Channel Type β-CD Dimers Induced by Dodecane. Sci Rep 4, 7533 (2014). https://doi.org/10.1038/srep07533
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep07533
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.