Figure 7
From: Tulane virus recognizes sialic acids as cellular receptors
Expression of SGCs and Tulane virus (TV) infection of LLC-MK2 cells.
(A) LLC-MK2 cells were stained with fluorescein-labeled Maackia amurensis leukoagglutinin (MAL, green). (B) Cells were stained via the same procedure as (A) without MAL (negative control). (C and D) LLC-MK2 cells at 15 hours post-TV infection were stained by fluorescein-labeled MAL (green) and hyperimmune rabbit serum against TV (red). MAL bound all cells and all TV-infected cells express SAs. Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).
