Figure 1
From: Opportunistic pathogen Candida albicans elicits a temporal response in primary human mast cells
C. albicans induced mast cell degranulation and cytokine release in a MOI-dependent manner.
(A) HMC-1 cells were infected with opsonized C. albicans yeasts (MOI 0.1, 1 and 10) for 1 hour, after which ß-hexosaminidase release was measured from supernatants of infection. (B–F) Shown are 5 cytokines at 6, 12 and 24 h post infection that were released differentially from different supernatants of mast cells infected with C. albicans (MOI 0.1 and 1) or of mast cells left uninfected. ß–hexosaminidase percentage release was defined by the amount of ß-hexosaminidase release from infected cell divided by spontaneous ß-hexosaminidase release from uninfected cells (% of ß –hexosaminidase release/% of ß –hexosaminidase control). Significance for (A–F) was analysed by Tukey one-way ANOVA *P ≤ 0.05. Data are presented as means of n = 4 (4) ± SD (ß-hexosaminidase release assay) and n = 3 (3) ± SD (Cytokine Multiplex).
