Table 1 Epitopes and characteristics of anti-human α subunit-blocking mAbs against RGD-integrin subfamily sequences.

From: Epitopes in α8β1 and other RGD-binding integrins delineate classes of integrin-blocking antibodies and major binding loops in α subunits

Antibody

Host

Binding site

Key residuesa (human)

Glycan recognition

Reference

αIIb

 LJ-CP8

Mouse

W3:41

156VEND159

No

7

 LJ-P9

Mouse

W2:41

79VGSQTL84

No

7

 10E5b

Mouse

W2:41

77RNVGSQ82

No

11

W3:34

R208, L213, H215

αv

 17E6c

Mouse

W3:41

Q145

No

14

W3:34

K203

 L230

Mouse

β-propeller

ND

ND

Unpublished

α5

 JBS5

Mouse

W2:41

S85

No

16

 mAb16d

Mouse

W2:23

E126, L128

No

16

W3:41

S156, W15744

44

W3:34

I210 45

45

 P1D6e

Mouse

W3:34

Y208, I210, L212

No

16

 SNAKA52

Mouse

W2:41

S85

No

16

α8

 YZ3 (and 3 related clones)

Chicken

W2:41

R82, K87

Yes (support binding)

This study

 YZ5

Chicken

W2:41

84NGT86, K87, S94

Yes (essential for binding)

This study

W3:34

K205/D206

 YZ26

Chicken

W2:41

K87

Yes (support binding)

This study

  1. aUnderline represents conserved residues with hosts.
  2. bDetermined according to atomic co-ordinations and not confirmed by mutagenesis experiments.
  3. cDetermined according to atomic co-ordinations and confirmed by mutagenesis experiments.
  4. dmAb16 is suggested to have an additional binding site on the β subunit46.
  5. eP1D6 blocks the synergy site but does not block binding of RGD-peptides to the binding pocket.
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