Figure 3
Mamld1 expression in pregnant WT mice.
(a) Mamld1 expression in whole ovaries from pregnant WT mice at 16.5 (n = 3), 17.5 (n = 5) and 18.5 dpc (n = 8). mRNA levels relative to that of Gapdh are shown. The results are expressed as the mean ± SEM. The average of mRNA levels at 16.5 dpc was defined as 1.0. Asterisks indicate statistical significance. (b–d) Mamld1 expression in corpora lutea and follicles. Arrowheads indicate Mamld1 signals in corpus luteum. Scale bars: 200 μm (b), 100 μm (c) and 20 μm (d). No specific expression of the negative control (a sense probe). CL, corpus luteum. (e) Known factors involved in functional luteolysis. Arrow and bar headed lines indicate stimulatory and inhibitory effects, respectively. Double lines indicate protein-receptor bindings. FP, prostaglandin F2α receptor; PRL, prolactin; PRLR, PRL receptor. (f) Gene expression in the whole ovaries in pregnant WT mice at 17.5 and 18.5 dpc (n = 5 and 8, respectively). The average of mRNA levels at 17.5 dpc was defined as 1.0. (g) Mamld1 expression in the whole ovaries (Ovary, n = 8) and uteri (Uterus, n = 4) from pregnant WT mice at 18.5 dpc. The average of mRNA levels in the whole ovaries was defined as 1.0.
