Table 2 BDNF ELISA kits performance.

From: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

Number of plates tested: 2

Aviscera-Bioscience (n = 38)

Biosensis (n = 39)

Millipore ChemiKineTM(n = 39)

Millipore Milliplex® (n = 38)

Promega Emax® (n = 40)

R&D System Quantikine® (n = 40)

Range ng/ml: min-max

14.1–32.4

13.6–41.4

14.5–58.5

8.7–32.4

12.1–27.5

15.4–46.2

Median ng/ml (25%–75%)

20.7 (18.5–24.4)

25.4 (21.5–32.0)

23.0 (18.6–25.9)

18.2 (14.2–22.2)

19.0 (16.9–21.7)

25.5 (21.0–30.5)

Declared intra-assay CV

4–6%

2–6%

4%

< 10%

2–9%

4–6%

Tested intra-assay CV

2%–3%

1%–1%

2%–3%

13%–14%

3%–7%

5%–6%

Declared inter-assay CV

8–10%

5–8%

9%

<10%

<9%

8–11%

Tested inter-assay CV

12%

5%

13%

18%

16%

20%

Tested processing time

7 hours

4 hours

22 hours

20 hours

24 hours

5 hours

  1. Comparison between declared performances and actual tested performances of the specified BDNF ELISA kits. Median and range values, intra- and inter-assay coefficients of variation (CV) were assessed by measuring BDNF serum level from healthy volunteers (n = 38–40). All the kits, except Milliplex®, which is based on multiplex technology (Luminex®/xMAP®), are based on classical sandwich ELISA.
Back to article page