Figure 5
Histological analysis of scar formation of the healed wounds 14 days after treatment.
(a) Representative images of wound tissues with HE staining (40×, scale bars 200 μm). (b) Representative images of wound tissues with Masson’s trichrome staining (40×, scale bars 200 μm); the scar tissues stained blue. (c) Quantitative data on the area, width and thickness of scars based on the images of tissue sections with Masson’s trichrome staining. (d) Representative images of wound tissues with Picrosirius red staining for analysis of collagen fibers. The inserted high-magnification images in the lower panel were used to detect the alignment of type I collagen (yellow) and type III collagen (green) in the scar using polarized light microscopy.
