Table 2 Primer sets used for amplification of TYLCV in this study.
From: Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes
Primer name | Sequence (5′ – 3′) | Target size |
|---|---|---|
Detection PCR | ||
TYLCV-F | GATGGCCGCGCCTTTTCCTTTTATGTGG | 390 bp |
TYLCV-R | GCTGCTGTATGGGCTGTCGAAGTTCAG | |
Real-time PCR | ||
TYLCV-C1-F | GCTCGTAGAGGGTGACGAA | 164 bp |
TYLCV-C1-R | CACAAAGTACGGGAAGCCCA | |
GAPDH-F | GCCACTCAGAAGACCGTTGA | 188 bp |
GAPDH-R | AGGTCAACCACGGACACATC | |
Full length sequencing | ||
TYLCV-FL-F | CTTACTTATACCTGGACACCTAATGGC | 2774 bp |
TYLCV-FL-R | ACACCGATACACCGATTGCCATAG | |
