Table 1 Functional and structural avidities of the A2/MART1 TCRs.

From: CD4+ and CD8+ TCRβ repertoires possess different potentials to generate extraordinarily high-avidity T cells

Clone

T cells derived from

TRBV

CDR3β

TRBJ

Structural avidity* without CD8

Structural avidity with CD8

EC50(μg/ml)

EC50(μg/ml)

Functional avidity† without CD8

Functional avidity with CD8

Cl.7E

CD4

27

CASSRDFGNTIYF

1–3

0.010

0.006

0.102

0.071

Cl.3P

CD4

5–1

CASSLTGGYGYTF

1–2

0.013

0.012

0.116

0.058

Cl.830

CD8

27

CASSLGGAYEQYF

2–7

0.013

0.008

0.056

0.036

Cl.8H

CD4

27

CASSPLGAMEQYF

2–7

0.014

0.008

0.063

0.040

Cl.1606

CD8

27

CASSLLGSYEQYF

2–7

0.015

0.008

0.031

0.031

Cl.7Q

CD4

27

CASSPYMMNTEAFF

1–1

0.016

0.009

0.065

0.043

Cl.1593

CD8

27

CASGNNQPQHF

1–5

0.016

0.008

0.104

0.047

Cl.9J

CD4

5–1

CASSWTGDGYTF

1–2

0.018

0.010

0.075

0.071

Cl.2J

CD4

27

CAASMGQGFGEQFF

2–1

0.018

0.008

0.100

0.072

Cl.2E

CD4

27

CASSWDWGNIQYF

2–4

0.019

0.014

0.070

0.056

Cl.11E

CD4

2

CASSVMAPLHF

1–6

0.022

0.011

0.121

0.074

DMF5‡

CD8

6–4

CASSLSFGTEAFF

1–1

0.022

0.013

0.363

0.102

Cl.4D

CD4

5–1

CASSWAGTGSEQYF

2–7

0.030

0.022

0.179

0.073

Cl.6X

CD4

2

CATGVTDTQYF

2–3

0.039

0.018

0.150

0.083

Cl.10V

CD4

5–1

CASSLQGANGELFF

2–2

0.043

0.033

0.405

0.140

Cl.6B

CD4

2

CASSEVAWQFF

2–1

0.044

0.013

0.600

0.120

Cl.1086

CD8

27

CASSLHGPGGYTF

1–2

0.048

0.013

0.554

0.076

Cl.3J

CD4

5–1

CASSLMGTEAFF

1–1

0.050

0.025

1.451

0.263

Cl.1599

CD8

27

CASSFLGAMAEAFF

1–1

0.069

0.018

0.053

0.032

Cl.6I

CD4

2

CATGRGATQYF

2–3

0.070

0.020

0.665

0.125

Cl.788

CD8

27

CASGPSYEQYF

2–7

0.071

0.013

0.880

0.121

Cl.794

CD8

27

CASSLLGDYGYTF

1–2

0.082

0.018

0.067

0.043

Cl.11C

CD4

2

CASDEGFGYTF

1–2

0.108

0.018

2.460

0.292

Cl.1574

CD8

27

CASSPWERINTEAFF

1–1

0.110

0.021

0.076

0.052

Cl.758

CD8

27

CASSPRLAGDGELFF

2–2

1.610

0.041

0.342

0.096

Cl.523

CD8

27

CASGSYEQYF

2–7

n.m

0.064

n.m

0.748

Cl.413

CD8

27

CASSVFGGDMGEKLFF

1–4

n.m

0.303

n.m

2.337

  1. *Structural avidity, expressed as EC50 in μg/ml, was defined as the concentration of A2/MART1 multimer required to achieve 50% of maximal multimer staining.
  2. †Functional avidity, expressed as EC50 in μg/ml, was defined as the concentration of peptide required to achieve 50% of maximal response.
  3. ‡DMF5 is a high affinity A2/MART1 TCR reported by Johnson et al.28 n.m, not measurable.
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