Figure 3
From: Activation of endothelial β-catenin signaling induces heart failure
Endothelial Nrg1 expression and cardiac ErbB signaling are suppressed in Bmx/CA mice.
(a) Quantitative RT-PCR analysis of Nrg1 expression in cardiac ECs isolated from Bmx/CA mice (Bmx/CA ECs) compared with ECs isolated from Ctrl mice (Ctrl ECs). **P < 0.01 (n = 4). (b) Quantitative RT-PCR analysis of Nrg1 and Axin2 expression in HCAECs stimulated with recombinant human Wnt3a (200 ng/mL) (Wnt-3a) or PBS containing 0.1% BSA (Ctrl). **P < 0.01, *P < 0.05 (n = 4). (c) Immunoprecipitation and western blotting analysis of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Ctrl or Bmx/CA mice at 1 week after TAM were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti-phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Bottom panel). *P < 0.05 (n = 4).
