Figure 4
From: Activation of endothelial β-catenin signaling induces heart failure
Administration of NRG1 rescued the cardiac phenotypes of Bmx/CA mice.
(a) Immunoprecipitation and western blotting analyses of ErbB2 and ErbB4 phosphorylation. Lysates from the heart of Bmx/CA treated with or without rNRG were precipitated with anti-ErbB2 or anti-ErbB4 antibody and the precipitates were blotted with anti-phosphorylated tyrosine antibody (pTyr (PY99)) or with the antibody used for precipitation. Phosphorylation levels were analyzed from the band density using ImageJ (Right panel). *P < 0.05 (n = 4). (b) Cardiac function of Ctrl mice (Ctrl) and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG) were evaluated by echocardiography before (Pre), 3rd and 5th day of TAM treatment (Tam3 and Tam5) and at the indicated time point after the 5 day- TAM treatment. **P < 0.01 versus Ctrl, ††P < 0.01 versus Bmx/CA + rNRG, §P < 0.05 versus Ctrl. (c) Representative electron microscopic images of the heart tissue from Ctrl and Bmx/CA mice treated with vehicle (Bmx/CA) or rNRG (Bmx/CA + rNRG). Scale bar: 2.5 μm. (d) Quantitation of vacuole formations. Vacuoles were evaluated as described in Fig. 2i. **P < 0.01.
