Table 2 Optimized ESI-MS and ESI-MS/MS parameters as obtained during method optimization.

From: Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells

Analyte

RT [min]

Precursor ion [m/z]

Ion species

Product ions a [m/z]

Relative intensity b

CE a,c [eV]

S-lens

DON

9.4

355.1

[M + Ac]−

265.2/247.2

29%

−17/−19

75

DON-3-sulfate

8.5

375.0

[M−H]−

345.0/247.0

59%

−21/−24

100

DON-15-sulfate

8.2

375.0

[M−H]−

97.0/163.1

22%

−35/−40

100

DON-3-glucuronide

8.8

471.1

[M−H]−

265.0/175.0/441.0

93%/37%

−27/−30/−23

150

DON-15-glucuronide

9.0

471.1

[M−H]−

265.0/175.0/441.0

27%/3%

−27/−30/−23

150

Deepoxy-DON

12.6

339.1

[M + Ac]−

249.0/59.0

106%

−15/−23

62

  1. aValues are given in the order quantifier ion/qualifier ion/qualifier ion 2 (in case of glucuronides).
  2. bSignal intensity of the qualifier transition in relation to the quantifier (qualifier/quantifier × 100).
  3. cCollision energy.
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