Figure 6 | Scientific Reports

Figure 6

From: Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity

Figure 6

cAMP, PKA, lipolysis, HSL, perilipin and ATGL in primary VAT and SAT adipocytes of Ctrl and OB rats.

(A) Effects of ISO on cAMP levels and relative PKA activity. (B) Effects of ISO and dbcAMP on lipolysis. (C) Effects of ISO and dbcAMP on HSL phosphorylation. (D) Effects of ISO and dbcAMP on perilipin phosphorylation. (E) Effects of ISO and dbcAMP on ATGL expression. The measurements were carried out after 2-h of incubation with BPS, ISO (10−6 mol/L), dbcAMP (10−3 mol/L) or ISO + dbcAMP. Values are mean ± S.E.M. *P < 0.05 vs. Ctrl; †P < 0.05 vs. PBS; ‡P < 0.05 vs. ISO; #P < 0.05 vs. VAT. n = 6 for each group in (A,B); n = 4 for each group in (C–E).

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