Figure 1

EBLN1-depleted cells accumulate DNA damage.

(A) Left and middle panels show representative immunofluorescence images of γH2AX foci in HeLa and MRC5A cells respectively, with the right panel showing quantification of γH2AX foci. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells). (B) Upper panel shows a representative western blot from HeLa cells demonstrating siRNA-mediated knockdown of EBLN1 with two individual siRNA. Additional examples of efficient EBLN1 depletion using these two siRNA are shown in Supplementary Figure S1A. Bottom panel shows representative γH2AX foci in control and individual EBLN siRNA-transfected HeLa cells, with the graph on right showing quantification of γH2AX foci. Data shown represents the mean from at least three independent experiments with associated SEMs (**p ≤ 0.01 compared to control siRNA cells). (C) Left panel shows representative immunofluorescence staining of 53BP1 foci in control and EBLN1-transfecetd HeLa cells. Graph on right shows mean percentage of 53BP1 positive cells. Data shown represents the mean from at least three independent experiments with associated SEMs (**p ≤ 0.01 compared to control siRNA cells). (D) Same as (C), except for pATM (Ser1981) foci in siRNA-transfected cells. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 compared to control siRNA cells). (E) Upper panel shows representative images of COMETs in control and EBLN1 siRNA-transfected HeLa cells. Lower panels show quantification of mean percentage DNA in COMET tails and mean COMET tail moment. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells).