Figure 5
Binding of [18F]-9 with AD homogenates (A,C) and Aβ1-42 fibrils (B,D). Aβ1-42fibrils and AD homogenates were incubated with increasing concentrations of [18F]-9. Representative plots of specific binding versus [18F]-9 concentration are shown for AD homogenates in (A) and Aβ fibrils in (B). Data points represent mean +/− standard deviations (n = 3). The data was analyzed by curve fitting to a one-site binding model using a nonlinear regression. Scatchard plots of binding are shown for AD homogenates (C) and Aβ1-42 fibrils (D) and are consistent with one-site binding model. Similar results were obtained in two independent experiments. Fluorescence binding assays of 5 with Aβ1-42 fibrils also indicated a saturable specific binding with a Kd = 1.58 ± 0.05 nM (SI, Fig. 2).
