Figure 4
From: Modeled changes of cerebellar activity in mutant mice are predictive of their learning impairments
Experimental and modeled Purkinje cell modulation is disrupted in the PC-Δγ2 mice.
(a) Part of cerebellar circuitry shown in Fig. 1a; Red “X” depicts severed connectivity between MLIs and PCs in the PC-Δγ2 mice. (b) Experimental and modeled eye movements in PC-Δγ2 mice as a function of training time of VOR phase-reversal training. Gain values (top panel) are normalized to the initial gain. Experimental data represent averages with SEM (blue shaded area) and SD (grey shaded area) of the PC-Δγ2 mice. Grey dotted line indicates the values of the littermate controls. Modeled changes are displayed for both gain and phase with SD (red line). (c) Linear regression plots displaying correlation between modeled and experimental data. (d) Representative PSTHs from in vivo recording in PC-Δγ2 during vestibular stimulation (0.6 Hz). (e) Polar plots of SS and CS modulation before and after learning (lighter and darker blue, respectively). The plots reveal much lower modulation amplitude than that of the controls. Note that some PCs did not modulate their SS activity (indicated with black arrows). Each dot represents a single cell. Phase of the modeled SS and CS are indicated with the arrows. (f) Modeled and experimentally measured modulation was initially lower than that of the controls and increased significantly following learning in the PC-Δγ2. The firing frequency was also significantly higher following learning both in experimental and modeled data. Error bars denote SD; *denote p < 0.05; **denote p < 0.001. (g) SS activity as a function of time in the cycle in a control (grey, initial value before learning; black, after training) and in the model with blocked MLI to PC inhibition (light blue, initial value before learning; dark blue, after training).
