Figure 1
Reduced efferocytosis of apoptotic cardiomyocytes in diabetic conditions.
(A) Fluorescent microscopy showing engulfment of apoptotic cells (AC; human ventricular cardiomyocytes, SV40) by THP1 cells (human monocyte cell line exposed to high glucose (35 mM glucose). (B) Quantitation of efferocytosis showing reduced efferocytosis by THP-1 cells in high glucose condition as compared to cells treated with normal glucose. n = 3, *P < 0.001. (C) Fluorescent microscopy showing engulfment of apoptotic cells (AC; human ventricular cardiomyocytes, SV40) by mouse bone marrow derived macrophages (BMM) from non-diabetic and diabetic (db/db) mice. (D) Quantitation of efferocytosis showing reduced efferocytosis by macrophages derived from diabetic (db/db) mice compared to cells from wild type (non-diabetic) mice. n = 3, **P < 0.001. Apoptotic cells are labeled with calcein (green) and macrophage nuclei stained with DAPI (blue). At least 100 macrophages were counted and the data represent three independent experiments done in triplicate.
