Figure 3
MerTK expression in RAW 264.7 cells exposed to high glucose.
(A) Immunoprecipitation and western blotting showing higher sMer (soluble form of MERTK) levels in the conditioned media of cells exposed to high glucose (HG) for 48 hrs compared to normoglycemia condition (NG). The corresponding cell lysate showing decrease in phosphorylated MerTK (p-Mer) levels in high glucose treated cells, while total MerTK (t-Mer) was unchanged. IgG antibody used as a negative control. (B) Densitometry of sMer in the above blot. (C) RAW 264.7 cells pretreated with ADAM blockers, genistein and ebselen, inhibit HG-induced increase in sMer and decrease in p-Mer. (D) Densitometry of sMer in the above blot, normalized to GAPDH expression.
